What is an Enzyme Linked Immunoassay (ELISA) Test?
The enzyme-linked immunoassay (ELISA) is used to identify antibodies in the blood produced by a harmful substance that has entered the body. This test is widely used to detect blood-borne viruses like HIV, HBV, HCV, and HTLV.
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Get A Second OpinionTypes of ELISA
There are four types of ELISA -
- Direct ELISA,
- Indirect ELISA,
- Sandwich ELISA, and
- Competitive ELISA.
Elisa Test Cost or Price in India
| Test Type | Blood Tests |
|---|---|
| Preparation | There is no special preparation for this test. The patient can eat and drink anything before the test. |
| Report | Within a day. |
| ELISA test costs in Hyderabad | Rs. 500 to Rs.1500 approximately. |
| ELISA test costs in Vizag | Rs. 500 to Rs.1500 approximately. |
| ELISA test costs in Nashik | Rs. 500 to Rs.1500 approximately |
| ELISA test costs in Aurangabad | Rs. 500 to Rs.1500 approximately |
| ELISA test costs in Nellore | Rs. 500 to Rs.1500 approximately |
| ELISA test costs in Chandanagar | Rs. 500 to Rs.1500 approximately |
| ELISA test costs in Srikakulam | Rs. 500 to Rs.1500 approximately |
| ELISA test costs in Sangamner | Rs. 500 to Rs.1500 approximately |
| ELISA test costs in Kurnool | Rs. 500 to Rs.1500 approximately |
| ELISA test costs in Kakinada | Rs. 500 to Rs.1500 approximately |
| ELISA test costs in Karimnagar | Rs. 500 to Rs.1500 approximately |
| ELISA test costs in Zaheerabad | Rs. 500 to Rs.1500 approximately |
| ELISA test costs in Sangareddy | Rs. 500 to Rs.1500 approximately |
| ELISA test costs in Nizamabad | Rs. 500 to Rs.1500 approximately |
| ELISA test costs in Mumbai | Rs. 500 to Rs.1500 approximately |
| ELISA test costs in Begumpet | Rs. 500 to Rs.1500 approximately |
| ELISA test costs in Vizianagram | Rs. 500 to Rs.1500 approximately |
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How to Prepare for an ELISA Test?
Steps to Prepare
Fasting:
Check if fasting is required; some tests may require 8-12 hours of fasting.
Medication Instructions:
- Consult your doctor regarding your current medications; some may need to be paused or adjusted.
- Bring a comprehensive list of all medications, supplements, and vitamins you are taking, including dosages and frequency.
Avoid Certain Substances:
- Refrain from consuming caffeine and alcohol for at least 24 hours before the test.
- Do not use tobacco products on the day of the test.
Hydration:
Ensure you drink plenty of water before the test to stay hydrated, unless instructed to fast completely.
Health Conditions:
Inform your doctor about any recent illnesses, infections, or vaccinations that could potentially affect the test results.
On the Day of the Test
Clothing:
Wear comfortable clothes with sleeves that can be easily rolled up.
Arrival:
Arrive at the testing facility slightly early to complete any necessary paperwork and to relax before the test.
Relaxation:
Try to remain calm and relaxed to avoid any impact on your blood pressure.
Communication:
Feel free to ask any last-minute questions or express any concerns to the healthcare professional conducting the test.
After the Test
Follow Post-Test Instructions:
- Adhere to any specific aftercare instructions provided by your healthcare provider.
- Resume your normal activities and diet unless advised otherwise.
Monitor for Side Effects:
Be vigilant for any adverse reactions at the blood draw site, such as excessive bruising or swelling, and contact your healthcare provider if necessary.
Findings of ELISA Test
- The ELISA test helps identify the situations that lead the immune system to produce antibodies, which the swab tests cannot quickly recognize.
- It helps find out about the viruses like HIV, HBV, HCV, and HTLV in the blood that lead the body to produce antibodies.
- You must visit a doctor for abnormal findings and get a complete diagnosis and treatment.
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Book an AppointmentFrequently Asked Questions
No, each ELISA can detect a specific antigen.
The plasma of the human body where the heterophilic antibodies are found can bind to both the capture and detection antibodies used in capturing ELISA by cross-linking with the assay antibodies, resulting in false-positive signals.
Typical ELISA test detection ranges from 0.1 to 1 fmole or 0.01ng to 0.1ng.
This test is recommended if you are exposed to HIV, as well as to diagnose other infectious conditions.
Horseradish peroxidase (HRP) and alkaline phosphatase (ALP) are commonly used enzymes.
The test can be accurate by 99.9% when used in combination with the confirmatory Western blot test.
Specific antibodies bind to the target antigen by detecting the presence of antigens, and to get the correct value, the plate must be coated with the antibodies with high affinity.
When a person has some infectious conditions, the test determines if the patient has antibodies developed due to any infection.
The ELISA test is sensitive due to the binding characteristics of the antibodies.
It requires a slight draw of blood, around 4 milliliters.
