What is the Widal Test?

  • The Widal test is a blood test used to confirm or rule out enteric fever, also known as typhoid fever.
  • The test typically shows the first signs of fever 6 to 30 days after exposure to the infection.
  • Doctors prescribe the Widal test for various reasons.
  • The test identifies antibodies against flagellar (H) and somatic (O) antigens that cause infection and typhoid fever.
  • The body produces specific antibodies when exposed to certain antigens.
  • The Widal test examines the interaction between these antigens and antibodies in patient blood samples.

What is the use of a Widal test?

There is a possibility of typhoid fever if you have symptoms such as high fever, lethargy,diarrhea,constipation, stomach discomfort, rose spot rashes,dry cough, and headache. Widal test is recommended by doctors in the diagnosis of typhoid illness. Typhoid fever is caused by the Salmonella enterica serotype typhi bacterium. The illness is caused by consuming contaminated food and drink containing feces. The Widal test detects typhoid-causing germs in the patient's bloodstream using an agglutination biochemical test.

Secure your health with a second opinion. Make informed decisions and book your appointment today!

Get A Second Opinion

Who Should Need To Get This Test Done?

If a patient exhibits signs of typhoid fever, the doctor may prescribe a Widal test to get a more accurate diagnosis. These are some of the symptoms:


How is the Widal Test Conducted?

There is no specific preparation necessary, and no fasting is required. A small needle is delicately inserted into a vein in the arm to get a blood sample. After that, the blood sample is sent to a laboratory for analysis.


Widal Testing Methods :

  • Qualitative Method : On the slides, one drop of undiluted blood serum is deposited, followed by one drop of O, H, AH, and BH antigens. The slide is gently shaken for a minute after the ingredients have been mixed using a separate applicator stick. If there is no agglutination, the test is declared negative.
  • Quantitative Approach : Following a positive qualitative test, a semi-procedural Widal tube test is performed, which comprises evaluating the positive antigens with a blood sample to confirm slide agglutination.

    Because of its higher sensitivity but poorer specificity, the slide Widal test should be used for screening, but positive slide test findings should be validated by the tube Widal test.


Understanding Test Ranges and Results

The titer value can be used to analyze a Widal test since it requires detailed and exact interpretation. To achieve a typhoid test result, titers ranging from 1:20, 1:40, 1:60, 1:80, 1:160, and 1:200 must be included in the diagnosis.

Negative Result - When the value of the O and H antigens is less than 1:160, the result is called negative and is typically regarded to be within the normal range for a Widal test.

Positive result - A positive result is present when the titer of O and H antigens is greater than 1:60 to 1:320. A positive Widal test result indicates that salmonella typhi is present in the blood.

The Widal test is a brief and affordable method to screen for typhoid illness, even in remote areas where medical care is limited.

Typhoid fever is increasing in urban and rural areas of developing nations due to poor sanitation and a lack of safe drinking water. However, the sickness can be readily managed if the typhoid patient is examined promptly and carefully cared for with a specialized diet and frequent treatment.

Ready to take control of your health journey? Book your appointment now and start your path towards wellness today!

Book an Appointment

Frequently Asked Questions

Which fevers can be diagnosed by the Widal test? The Widal test is used to diagnose typhoid fever.

Positive Widal test results suggest a bacterial infection with Salmonella enterica serovar typhi in the blood. The positive report indicates active typhoid fever infection.

A negative Widal test indicates that a person does not have an enteric fever (Typhoid and Paratyphoid fever) infection.

A Widal test should be done 1 week after the infection.

It is not necessary to take a Widal test on an empty stomach.

After seven to ten days of contracting the infection, the Widal test is positive.

The Widal test is a slide agglutination test, and blood serum is taken for the sample to test.

Two types of methods can be followed for a Widal test, which are- Slide methodsTube methods

With proper treatment, typhoid fever symptoms improve within 3 to 6 days.

Yes, It is possible to have typhoid without experiencing fever or any other symptoms or indicators of infection.

No, there is no risk related to the Widal test.

Avoiding contaminated foods and drinks can help avoid typhoid illness. In addition, adequate immunization against typhoid fever can help prevent any possible infection cases.

Book Doctor Appointment
Book Free Appointment
Make an appointment just in few minutes - Call Us Now
Whats app Health Packages Book an Appointment Second Opinion